![]() ![]() Potassium permanganate, counterstain renders tissue and its debris non-fluorescent, therefore reducing the possibility of artifacts. Principle of Auramine -rhodamine stainĪuramine-rhodamine is the fluorochrome dye that forms a complex with mycolic acids found in the acid-fast cell wall of organisms that resist decolorization by acid-alcohol. The acid fastness of Mycobacterium tuberculosis is due to having a thick cell wall composed of waxes and lipids that has a high content of mycolic acid. Ziehl-Neelsen (hot), Kinyoun (cold) are still widely used methods to detect acid-fast structures in these organisms in developing countries but sensitivity is high of fluorochrome stain. ![]() It is a fluorochrome stain and used to visualize acid-fast structures of various microorganisms especially Mycobacterium tuberculosis and in modified form for Mycobacterium leprae, Nocardia species, Cryptosporidium parvum, Cyclospora cayetanensis, Isospora belli and fungal spores. Auramine-Rhodamine Stain Introduction of auramine -rhodamine stain Lehman & George Manuselis, 3rd edition2007, Publisher Elsevier.Ģ. Simmous, 4th ed, Publisher Churchill Living Stone, New York, Melbourne, Sans Franscisco 1996.
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